SHA-SRB Kits for Oil and Gas

The SHA-SRB single-use analysis kit detects the organisms most commonly associated with microbiologically influenced corrosion (MIC), hydrocarbon reservoir souring, and biofouling in oil and gas operations. Molecular probes in this kit provide a taxonomic hierarchy to facilitate species identification of the most prevalent organisms but also higher taxa to assure functional SRB coverage.

The supervising analyst can deduce organism content by subtracting more specific components in the semi-quantitative report from approximate abundance of higher taxonomic orders. Semi-quantitation also provides vital data for assessing biocide effectiveness and time trends in individual wells.

THE SHA-SRB KIT IS AN ALPHA-STAGE DEVELOPMENT PRODUCT.

SRB Probes, Available

Desulfovibrio desulfuricans

The dominant SRB species associated with MIC in the oil and gas industry.

Desulfovibrio spp.

Multiple species of the Desulfovibrio genus, including D. desulfuricans.

Deltaproteobacteria

Multiple genera of Deltaproteobacteria to broaden total SRB coverage.

In Development

Additional specific family, genera, and species important in the oil and gas industry.

Pan-Bacteria Probe

Essentially all bacteria in a sample.

Pan-Euryarchaeota Probe

Broad range of Archaea.

Kit Contents

Single-Use Components

  • 10 each: Pre-coated 12-prong strips with customer-specified organism probes to support customer-specified number of target prongs, replicates, and controls (store at 2º-8º C)

  • 10 each: 96-well microtiter plates, prefilled with reagents (store at 2º-8º C)

    • Hapten-labeled signal probe

    • ​Anti-hapten horseradish peroxidase enzyme (HRP) conjugate for signal development

    • TMB reporter dye for visual or instrumented assessment (650 nm)

    • Sulfuric acid for higher sensitivity TMB spectrometric reading (450 nm)


Additional Reagent​

  • 10 vials of lysis buffer​​, each sufficient for 20 sample filters
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